CXXC1 Polyclonal antibody

CXXC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27963-1-AP

Synonyms

2410002I16Rik, 5830420C16Rik, CFP1, CGBP, CpG binding protein, CXXC finger 1 (PHD domain), CXXC1, hCGBP, HsT2645, PCCX1, PHF18, SPP1

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Validation Data Gallery

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  WB analysis of K-562 using 27963-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27963-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27963-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27963-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27963-1-AP (CXXC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27963-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27963-1-AP (CXXC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27963-1-AP targets CXXC1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXXC1 fusion protein Ag27558
• Full Name: CXXC finger 1 (PHD domain)
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC015733
• Gene Symbol: CXXC1
• Gene ID (NCBI): 30827
• RRID: AB_2881023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.