Tested Applications
| Positive WB detected in | MCF-7 cells, mouse brain tissue, SH-SY5Y cells |
| Positive IP detected in | SH-SY5Y cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16513-1-AP targets CXXC5 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9733 Product name: Recombinant human CXXC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC017439 Sequence: MMGGESADKATAAAAAASLLANGHDLAAAMAVDKSNPTSKHKSGAVASLLSKAERATELAAEGQLTLQQFAQSTEMLKRVVQEHLPLMSEAGAGLPDMEAVAGAEALNGQSDFPYLGAFPINPGLFIMTPAGVFLAESALHMAGLAEYPMQGELASAISSGKKKRKRCGMCAPCRRRINCEQCSSCRNRKTGHQICKFRKCEELKKKPSAALEKVMLPTGAAFRWFQ Predict reactive species |
| Full Name | CXXC finger 5 |
| Calculated Molecular Weight | 322aa,33 kDa; 227aa,24 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC017439 |
| Gene Symbol | CXXC5 |
| Gene ID (NCBI) | 51523 |
| RRID | AB_2878269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7LFL8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CXXC5 antibody 16513-1-AP | Download protocol |
| IHC protocol for CXXC5 antibody 16513-1-AP | Download protocol |
| IP protocol for CXXC5 antibody 16513-1-AP | Download protocol |
| WB protocol for CXXC5 antibody 16513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CXXC5 antibody (Cat# 16513-1-AP) has 11 citations spanning journals including Nature Communications, Advanced Science, Journal of Experimental Medicine, Oncogene, Haematologica, Protein Science, Frontiers in Cell and Developmental Biology, Chemical & Biological Interactions, Scientific Reports, Journal of Biological Chemistry, and International Journal of Cardiology. Research fields cover prostate cancer, ferroptosis, epigenetic regulation, immune response, hepatocellular carcinoma, erythropoiesis, protein degradation, breast carcinogenesis, and cardiac development.
| Species | Application | Title |
|---|---|---|
Nat Commun A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.
| ||
Adv Sci (Weinh) ZDHHC2-Dependent Palmitoylation Dictates Ferroptosis and Castration Sensitivity in Prostate Cancer via Controlling ACSL4 Degradation and Lipid Peroxidation | ||
J Exp Med Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.
| ||
Oncogene NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.
| ||
Haematologica The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion. | ||
Protein Sci CXXC5 is a ubiquitinated protein and is degraded by the ubiquitin-proteasome pathway |
