CXorf26 Polyclonal antibody

CXorf26 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20137-1-AP

Synonyms

PBDC1, UPF0368 protein Cxorf26, Polysaccharide biosynthesis domain-containing protein 1

---

Validation Data Gallery

• 

  WB analysis of A549 using 20137-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20137-1-AP (CXorf26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20137-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20137-1-AP (CXorf26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20137-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20137-1-AP (CXorf26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20137-1-AP (CXorf26 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20137-1-AP (CXorf26 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 20137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20137-1-AP (CXorf26 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20137-1-AP (CXorf26 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 20137-1-AP

  Immunofluorescent analysis of HeLa cells using 20137-1-AP (CXorf26 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse lung tissue
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20137-1-AP targets CXorf26 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXorf26 fusion protein Ag14089
• Full Name: chromosome X open reading frame 26
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 26-35 kDa
• GenBank Accession Number: BC001220
• Gene Symbol: CXorf26
• Gene ID (NCBI): 51260
• RRID: AB_2878644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.