PIH1D3 Polyclonal antibody

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  IHC staining of human lung using 25309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25309-1-AP (PIH1D3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 25309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25309-1-AP (PIH1D3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 25309-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25309-1-AP (PIH1D3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 25309-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25309-1-AP (PIH1D3 antibody) at dilution of 1:200 (under 40x lens).

PIH1D3 Polyclonal Antibody for IHC, ELISA

Cat No. 25309-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

CXorf41, PIH1D3, Sarcoma antigen NY SAR 97

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Tested Applications

• Positive IHC detected in: human lung tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

25309-1-AP targets PIH1D3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIH1D3 fusion protein Ag15039
• Full Name: chromosome X open reading frame 41
• Calculated Molecular Weight: 214 aa, 24 kDa
• GenBank Accession Number: BC033510
• Gene Symbol: DNAAF6
• Gene ID (NCBI): 139212
• RRID: AB_2880024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IHC,IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang