CXorf56 Polyclonal antibody

CXorf56 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24021-1-AP

Synonyms

CXorf56, STEEP, STING ER exit protein, UPF0428 protein CXorf56

---

Validation Data Gallery

• 

  WB analysis of HeLa using 24021-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24021-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24021-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 24021-1-AP

  IP result of anti-CXorf56 (IP:24021-1-AP, 4ug; Detection:24021-1-AP 1:1000) with mouse kidney tissue lysate 8000ug.

• 

  IF Staining of HeLa using 24021-1-AP

  Immunofluorescent analysis of HeLa cells using 24021-1-AP (CXorf56 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

The immunogen of 24021-1-AP is CXorf56 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXorf56 fusion protein Ag19856
• Full Name: chromosome X open reading frame 56
• Calculated Molecular Weight: 222 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC023506
• Gene Symbol: CXorf56
• Gene ID (NCBI): 63932
• RRID: AB_2879404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CxORF56 (also known as STEEP) is an evolutionarily highly conserved protein whose function is unknown. Recently CxORF56 has been identified as a protein that interacts with STING, and it is essential for STING ER exit. CxORF56-dependent STING ER exit is essential for STING signaling in antiviral defense and in inflammatory diseases.

Publications

Species	Application	Title
human	WB	EBioMedicine; A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology.; Authors - Thaneas Prabakaran
monkey	WB	J Virol; The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1; Authors - Feifei Diao
human	WB,IP	NPJ Breast Cancer; Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer; Authors - Ying Zhu
human	WB	Nat Commun; Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds; Authors - Bao-Cun Zhang