CYB5B Polyclonal antibody

• Featured Product
• KD/KO Validated

CYB5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15469-1-AP

Synonyms

OMB5, Cytochrome b5 type B, CYPB5M, CYB5M, CYB5 M

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Validation Data Gallery

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  WB analysis using 15469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.

• 

  WB analysis using 15469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15469-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15469-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15469-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15469-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15469-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 15469-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15469-1-AP (CYB5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 15469-1-AP

  IP result of anti-CYB5B (IP:15469-1-AP, 3ug; Detection:15469-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15469-1-AP (CYB5B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15469-1-AP (CYB5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15469-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15469-1-AP (CYB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, human liver tissue, mouse lung tissue, human adrenal gland tissue, rat liver tissue, rat lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

15469-1-AP targets CYB5B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYB5B fusion protein Ag7771
• Full Name: cytochrome b5 type B (outer mitochondrial membrane)
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 20-21 kDa
• GenBank Accession Number: BC004373
• Gene Symbol: CYB5B
• Gene ID (NCBI): 80777
• RRID: AB_2230349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYB5B(Cytochrome b5 type B) is also named as CYB5M, OMB5 and belongs to the cytochrome b5 family. It is a 21 kDa membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. This protein is expressed on the plasma membrane of lymphoma cells but not normal lymphocytes, reactive lymphocytes or bone marrow precursor cells(PMID:20100355). The full length protein has a propeptide with 11 amino acids.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.; Authors - Paige Dickson ; KD Validated
mouse	WB	PLoS Genet; Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse; Authors - Eriks Smagris
human	IHC	Front Mol Biosci; Comprehensive Analysis of mTORC1 Signaling Pathway-Related Genes in the Prognosis of HNSCC and the Response to Chemotherapy and Immunotherapy.; Authors - Zhao Ding
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková
human	WB	J Cell Biol; Mechanosensitive super-enhancers regulate genes linked to atherosclerosis in endothelial cells; Authors - Jin Li