CYB5R1 Polyclonal antibody

CYB5R1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11807-1-AP

Synonyms

B5R.1, CYB5R1, cytochrome b5 reductase 1, humb5R2, NADH cytochrome b5 reductase 1, NQO3A2

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Validation Data Gallery

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  WB analysis of human placenta using 11807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11807-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11807-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 11807-1-AP

  IP result of anti-CYB5R1 (IP:11807-1-AP, 4ug; Detection:11807-1-AP 1:1000) with human placenta tissue lysate 1520ug.

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  IHC staining of human breast cancer using 11807-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11807-1-AP (CYB5R1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of PC-3 using 11807-1-AP

  Immunofluorescent analysis of PC-3 cells using 11807-1-AP (CYB5R1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, human brain tissue, PC-3 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11807-1-AP targets CYB5R1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYB5R1 fusion protein Ag2475
• Full Name: cytochrome b5 reductase 1
• Calculated Molecular Weight: 305 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC018732
• Gene Symbol: CYB5R1
• Gene ID (NCBI): 51706
• RRID: AB_2089968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Free Radic Res; Iron deficiency aggravates DMNQ-induced cytotoxicity via redox cycling in kidney-derived cells; Authors - Daisaku Yoshihara
human	WB	J Transl Med; Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy; Authors - Tingting Zhao