Tested Applications
| Positive WB detected in | human placenta tissue, human brain tissue, PC-3 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11807-1-AP targets CYB5R1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2475 Product name: Recombinant human CYB5R1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC018732 Sequence: MGIQTSPVLLASLGVGLVTLLGLAVGSYLVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPTAHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKVGDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGPPPMVQLACHPNLDKLGYSQKMRFTY Predict reactive species |
| Full Name | cytochrome b5 reductase 1 |
| Calculated Molecular Weight | 305 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC018732 |
| Gene Symbol | CYB5R1 |
| Gene ID (NCBI) | 51706 |
| RRID | AB_2089968 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHQ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| IHC protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| IP protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| WB protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYB5R1 Polyclonal antibody (Cat# 11807-1-AP) has 4 citations. These appear in J Transl Med, Free Radic Biol Med, Free Radic Res, and Adv Sci. The associated research fields include diabetic nephropathy and alternative polyadenylation, ferroptosis and redox homeostasis, iron deficiency–induced cytotoxicity in kidney cells, and lyssavirus-mediated suppression of ferroptosis during viral replication.
| Species | Application | Title |
|---|---|---|
J Transl Med Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy | ||
Free Radic Biol Med Downregulation of the SREBP pathways and disruption of redox status by 25-hydroxycholesterol predispose cells to ferroptosis
| ||
Free Radic Res Iron deficiency aggravates DMNQ-induced cytotoxicity via redox cycling in kidney-derived cells | ||
Adv Sci (Weinh) Lyssavirus Nucleoprotein Induces GPAT4-Mediated CYB5R1 Depalmitoylation to Suppress Ferroptosis and Promote Viral Replication. |
