CYB5R4 Polyclonal antibody

CYB5R4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12626-1-AP

Synonyms

b5+b5R, cb5/cb5R, CYB5R4, cytochrome b5 reductase 4, Flavohemoprotein b5/b5R, NCB5OR, RP4 676J13.1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 12626-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12626-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12626-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12626-1-AP (CYB5R4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12626-1-AP targets CYB5R4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYB5R4 fusion protein Ag3318
• Full Name: cytochrome b5 reductase 4
• Calculated Molecular Weight: 55.8 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC025380
• Gene Symbol: CYB5R4
• Gene ID (NCBI): 51167
• RRID: AB_2090295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Redox Biol; Cytochrome b5 reductase is the component from neuronal synaptic plasma membrane vesicles that generates superoxide anion upon stimulation by cytochrome c.; Authors - Alejandro K Samhan-Arias