Tested Applications
| Positive WB detected in | human adrenal gland tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18729-1-AP targets CYLC2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cylicin, basic protein of sperm head cytoskeleton 2 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC114547 |
| Gene Symbol | CYLC2 |
| Gene ID (NCBI) | 1539 |
| RRID | AB_2276962 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYLC2, also named as CYL2, possible plays an architectural role during spermatogenesis. It may be involved in spermatid differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYLC2 antibody 18729-1-AP | Download protocol |
| WB protocol for CYLC2 antibody 18729-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYLC2 Polyclonal antibody (Cat# 18729-1-AP) has 1 total citation. It was cited in Ecotoxicology and Environmental Safety (IF 6.6), in a 2026 study investigating how PFOA exposure impairs sperm head shaping in mice, reducing acrosome reaction and increasing DNA oxidative damage. The research field encompasses reproductive toxicology and environmental health, with the antibody applied via Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. |





