CYP11A1 Polyclonal antibody

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• CYP11A1 Polyclonal antibody (13363-1-AP)

CYP11A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13363-1-AP Copied!

Publications (102)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cholesterol desmolase, Cholesterol side-chain cleavage enzyme, mitochondrial, CYP11A, CYPXIA1, Cytochrome P450 11A1

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  WB analysis using 13363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 13363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 13363-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 13363-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP11A1 antibody (13363-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human placenta tissue, HeLa cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13363-1-AP targets CYP11A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4182

  Product name: Recombinant human CYP11A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC032329

  Sequence: MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRG

• Full Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Calculated Molecular Weight: 521 aa, 60 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC032329
• Gene Symbol: CYP11A1
• Gene ID (NCBI): 1583
• RRID: AB_2088552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05108
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP11A1 is also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. It is the first enzyme of all steroidogenic pathways, its elimination results in syndromes of steroid deficiency, including electrolyte imbalance, aberrant gene regulation, and suppression of male sex organ development(PMID: 12145347). Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al. The murine 32-kDa isoform of Cyp11a1 is not produced by omission of the first ATG-containing exon (as is the case for human CYP11A1) but perhaps by alternative translational initiation(PMID:19342447).

Publications

What published studies show

The CYP11A1 Polyclonal antibody (Cat# 13363-1-AP) has accumulated 102 citations published in over 60 journals, including Science, Nature Communications, Nature Aging, Cancer Research, Autophagy, Journal of Clinical Investigation, Free Radical Biology and Medicine, and Reproductive Biology and Endocrinology. Associated research fields encompass steroidogenesis, reproductive endocrinology, Leydig/granulosa/Sertoli cell biology, oxidative stress, ferroptosis, ovarian and testicular aging, environmental toxicology, PCOS, mitochondrial function, and cancer biology. Primary applications include Western blot, immunohistochemistry, and immunofluorescence.

Automatically generated from the 102 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Science; Pregnenolone can protect the brain from cannabis intoxication.; Authors - Monique Vallée
human	IF	Nat Aging; Targeting dysregulated phago-/auto-lysosomes in Sertoli cells to ameliorate late-onset hypogonadism; Authors - Zhiwen Deng
	WB	Cancer Res; ANO1 reprograms cholesterol metabolism and the tumor microenvironment to promote cancer metastasis; Authors - Chun-Miao Deng
mouse	WB	Autophagy; Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.; Authors - Wenwen Liu
chicken	WB	Nat Commun; Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens; Authors - Dandan Wang
human	WB	Nat Commun; 1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.; Authors - Michaela Schedel