CYP1B1 Monoclonal antibody

CYP1B1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1G8D4

Cat no : 67033-1-Ig

Synonyms

CP1B, CYP1B1, CYPIB1, Cytochrome P450 1B1, GLC3A, P4501B1



Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, rat brain tissue, mouse brain tissue, pig brain tissue, T-47D cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

67033-1-Ig targets CYP1B1 in WB,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP1B1 fusion protein Ag13380
Full Name cytochrome P450, family 1, subfamily B, polypeptide 1
Calculated molecular weight 61 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC012049
Gene symbol CYP1B1
Gene ID (NCBI) 1545
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Protocols

Product Specific Protocols
WB protocol for CYP1B1 antibody 67033-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols