CYP2C19 Polyclonal antibody

CYP2C19 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55453-1-AP

Synonyms

(R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPCJ, CYP2C, CYP2C19, CYPIIC17, CYPIIC19, Cytochrome P450 11A, Cytochrome P450 254C, Cytochrome P450 2C19, Mephenytoin 4 hydroxylase, P450C2C, P450IIC19

---

Validation Data Gallery

• 

  IHC staining of human liver cancer using 55453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55453-1-AP (CYP2C19 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 55453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55453-1-AP (CYP2C19 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 55453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 55453-1-AP (CYP2C19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55453-1-AP targets CYP2C19 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 2, subfamily C, polypeptide 19
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: NM_000769
• Gene Symbol: CYP2C19
• Gene ID (NCBI): 1557
• RRID: AB_2881340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.