CYP2D6 Monoclonal antibody

CYP2D6 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

8B11D12

Cat no : 60297-1-Ig

Synonyms

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1



Tested Applications

Positive WB detected inHepG2 cells, fetal human brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60297-1-Ig targets CYP2D6 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYP2D6 fusion protein Ag12605
Full Name cytochrome P450, family 2, subfamily D, polypeptide 6
Calculated molecular weight 497 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC075023
Gene symbol CYP2D6
Gene ID (NCBI) 1565
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for CYP2D6 antibody 60297-1-IgDownload protocol
IHC protocol for CYP2D6 antibody 60297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols