Tested Applications
| Positive WB detected in | mouse liver tissue, Jurkat cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19937-1-AP targets CYP2E1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 50-57 kDa |
| GenBank Accession Number | NM_000773 |
| Gene Symbol | CYP2E1 |
| Gene ID (NCBI) | 1571 |
| RRID | AB_10646444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05181 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP2E1-Specific antibody 19937-1-AP | Download protocol |
| IHC protocol for CYP2E1-Specific antibody 19937-1-AP | Download protocol |
| WB protocol for CYP2E1-Specific antibody 19937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYP2E1-Specific Polyclonal antibody (Cat# 19937-1-AP) has 144 citations. These appear across journals including Cell Metabolism, Nature Metabolism, Cell Host & Microbe, Nature Communications, Hepatology, Cell Discovery, Redox Biology, Food & Function, Molecules, Scientific Reports, and Food Chemistry & Toxicology. Research fields center on liver biology and toxicology: acetaminophen-induced liver injury, alcoholic liver disease, non-alcoholic fatty liver disease, liver fibrosis, oxidative stress, drug metabolism, and hepatocellular carcinoma. Applications are predominantly Western blot, with some IHC and IF.
| Species | Application | Title |
|---|---|---|
Nat Metab LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration | ||
Cell Host Microbe Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice | ||
Cell Host Microbe Oral magnesium prevents acetaminophen-induced acute liver injury by modulating microbial metabolism | ||
Cell Host Microbe Cohabitation facilitates microbiome shifts that promote isoflavone transformation to ameliorate liver injury | ||
Nat Commun The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury |
Reviews
What customers say
All three reviews are 5-star ratings for Western Blot applications. Users tested the antibody on mouse tissues including liver, brain, lung, and kidney, with dilutions ranging from 1:1,000 to 1:8,000. One reviewer confirmed a clear band at approximately 55 kDa in mouse liver lysates after overnight incubation at 4°C. Another noted successful detection of CYP2E1 in liver protein extracts. Overall, reviewers describe it as a great antibody for WB with reliable performance across multiple mouse tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (07-08-2026) | Great antibody for WB.
 |
Kamal (Verified Customer) (02-15-2024) | Mouse liver lysates were subjected to SDS PAGE followed by western blot with Cyp2e1 antibody at dilution of 1:5000 incubated at 4 degree overnight. Bands appeared at about 55 kDa.
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James (Verified Customer) (11-18-2022) | Detection of CYP2E1 in liver protein extracts
 |
