CYP2E1-Specific Monoclonal antibody, PBS Only

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  WB analysis of pig liver using 67263-1-Ig (same clone as 67263-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 67263-1-Ig (same clone as 67263-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67263-1-Ig (same clone as 67263-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CYP2E1-Specific antibody (67263-1-Ig, Clone: 1F12G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Cat No. 67263-1-PBS

Clone No.1F12G7

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, rat, pig

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Synonyms

CYP2E1, 1F12G7, 4-nitrophenol 2-hydroxylase, CPE1, CYP2E

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Product Information

67263-1-PBS targets CYP2E1-Specific in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with mouse, rat, pig samples.

• Tested Reactivity: mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: NM_000773
• Gene Symbol: CYP2E1
• Gene ID (NCBI): 1571
• RRID: AB_2882534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05181
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.