Tested Applications
| Positive WB detected in | HUVEC cells, L02 cells, mouse skeletal muscle tissue, mouse heart tissue, HepG2 cells, rat heart tissue |
| Positive IHC detected in | human liver tissue, mouse heart tissue, human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
13562-1-AP targets CYP2J2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4435 Product name: Recombinant human CYP2J2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 152-502 aa of BC032594 Sequence: KKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITISPVSHRLCAVPQV Predict reactive species |
| Full Name | cytochrome P450, family 2, subfamily J, polypeptide 2 |
| Calculated Molecular Weight | 502 aa, 58 kDa |
| Observed Molecular Weight | 58-65 kDa |
| GenBank Accession Number | BC032594 |
| Gene Symbol | CYP2J2 |
| Gene ID (NCBI) | 1573 |
| RRID | AB_2090013 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51589 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYP2J2(Cytochrome P450 2J2) belongs to the cytochrome P450 family. This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. The human CYP2J member, CYP2J2, is shown to be expressed in several tissues such as the small intestine, liver, heart, kidney, and lung and may play a role in the oxidative bioactivation of arachidonic acid(PMID:12386130).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP2J2 antibody 13562-1-AP | Download protocol |
| WB protocol for CYP2J2 antibody 13562-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Life Sci CYP2J2-produced epoxyeicosatrienoic acids attenuate ischemia/reperfusion-induced acute kidney injury by activating the SIRT1-FoxO3a pathway. | ||
Pharmacology Berberine Induces CYP2J2 Expression in Human U251 Glioma Cells via Regulation of Peroxisome Proliferator-Activated Receptor Alpha. | ||
PeerJ CYP2C11 played a significant role in down-regulating rat blood pressure under the challenge of a high-salt diet. | ||
Placenta Altered distribution of fatty acid exerting lipid metabolism and transport at the maternal-fetal interface in fetal growth restriction | ||
Cell Death Dis Epoxy metabolites of linoleic acid promote the development of breast cancer via orchestrating PLEC/NFκB1/CXCL9-mediated tumor growth and metastasis |
