CYP39A1 Polyclonal antibody

CYP39A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16062-1-AP

Synonyms

CYP39A1, Cytochrome P450 39A1, hCYP39A1, Oxysterol 7 alpha hydroxylase

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Validation Data Gallery

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  WB analysis of L02 using 16062-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16062-1-AP (CYP39A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 16062-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16062-1-AP (CYP39A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16062-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16062-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 16062-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16062-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16062-1-AP (CYP39A1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, SMMC-7721 cells
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16062-1-AP targets CYP39A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP39A1 fusion protein Ag8896
• Full Name: cytochrome P450, family 39, subfamily A, polypeptide 1
• Calculated Molecular Weight: 469 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010358
• Gene Symbol: CYP39A1
• Gene ID (NCBI): 51302
• RRID: AB_2878211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.