CYP3A5 Polyclonal antibody

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• CYP3A5 Polyclonal antibody (13737-1-AP)

CYP3A5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13737-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CP35, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3

• 

  WB analysis of mouse liver using 13737-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 13737-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 13737-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 13737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 13737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 13737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13737-1-AP targets CYP3A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4674

  Product name: Recombinant human CYP3A5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 152-502 aa of BC033862

  Sequence: YGDVLVRNLRREAEKGKPVTLKDIFGAYSMDVITGTSFGVNIDSLNNPQDPFVESTKKFLKFGFLDPLFLSIILFPFLTPVFEALNVSLFPKDTINFLSKSVNRMKKSRLNDKQKHRLDFLQLMIDSQNSKETESHKALSDLELAAQSIIFIFAGYETTSSVLSFTLYELATHPDVQQKLQKEIDAVLPNKAPPTYDAVVQMEYLDMVVNETLRLFPVAIRLERTCKKDVEINGVFIPKGSMVVIPTYALHHDPKYWTEPEEFRPERFSKKKDSIDPYIYTPFGTGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLDTQGLLQPEKPIVLKVDSRDGTLSGE

• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 5
• Calculated Molecular Weight: 502 aa, 57 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC033862
• Gene Symbol: CYP3A5
• Gene ID (NCBI): 1577
• RRID: AB_2090516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20815
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CYP3A5 polyclonal antibody (Cat# 13737-1-AP) has 3 total citations published in Int J Mol Sci, Front Oncol, and Oncotarget. The associated research fields include hepatocellular carcinoma prognostic signatures based on metabolic pathway phenotypes, gene expression reprogramming in liver cancer cells, and the role of alpha-ketoglutarate in suppressing NF-κB-mediated inflammation while enhancing PXR-regulated detoxification pathways.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; Gene Expression Reprogramming by Citrate Supplementation Reduces HepG2 Cell Migration and Invasion; Authors - Rocchina Miglionico
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
		Oncotarget; Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.; Authors - Liuqin He