CYP3A7 Polyclonal antibody

CYP3A7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55428-1-AP

Synonyms

CP37, CYP3A7, CYPIIIA7, Cytochrome P450 3A7, Cytochrome P450 HFLA, P450 HFLA

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Validation Data Gallery

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  WB analysis of L02 using 55428-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55428-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55428-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55428-1-AP (CYP3A7 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 55428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55428-1-AP (CYP3A7 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 55428-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A7 antibody (55428-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

55428-1-AP targets CYP3A7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 7
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_000765
• Gene Symbol: CYP3A7
• Gene ID (NCBI): 1551
• RRID: AB_2881328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Front Bioeng Biotechnol; Recapitulation of Human Embryonic Heartbeat to Promote Differentiation of Hepatic Endoderm to Hepatoblasts.; Authors - Koki Yoshimoto