CYP3A7 Polyclonal antibody

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• CYP3A7 Polyclonal antibody (55428-1-AP)

CYP3A7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55428-1-AP Copied!

Publications (1)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CP37, CYP3A7, CYPIIIA7, Cytochrome P450 3A7, Cytochrome P450 HFLA, P450 HFLA

• 

  WB analysis of L02 using 55428-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 55428-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 55428-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55428-1-AP (CYP3A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 55428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55428-1-AP (CYP3A7 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 55428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55428-1-AP (CYP3A7 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 55428-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A7 antibody (55428-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55428-1-AP targets CYP3A7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 7
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_000765
• Gene Symbol: CYP3A7
• Gene ID (NCBI): 1551
• RRID: AB_2881328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24462
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CYP3A7 Polyclonal antibody (Cat# 55428-1-AP) has 1 total citation. It appears in Frontiers in Bioengineering and Biotechnology, in a study on recapitulating human embryonic heartbeat to promote differentiation of hepatic endoderm to hepatoblasts. The associated research field is developmental bioengineering, specifically hepatic organoid differentiation.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Front Bioeng Biotechnol; Recapitulation of Human Embryonic Heartbeat to Promote Differentiation of Hepatic Endoderm to Hepatoblasts.; Authors - Koki Yoshimoto

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

One reviewer rated this antibody 5 out of 5 stars, describing it as a "very good antibody for flow cytometry." They used it at a 1:1000 dilution for both Western Blot and Flow Cytometry applications with lot 09000840, reporting positive results overall.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Sai Sindhura (Verified Customer) (01-08-2026)

VERY GOOD ANTIBODY FOR FLOW CYTOMETRY

• Applications: Western Blot, Flow Cytometry
• Primary Antibody Dilution: 1:1000