CYP4A11 Polyclonal antibody

CYP4A11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11688-1-AP

Synonyms

20 HETE synthase, CP4Y, CYP4A11, CYP4A2, CYP4AII, CYPIVA11, Cytochrome P 450HK omega, Cytochrome P450 4A11, Cytochrome P450HL omega, Fatty acid omega hydroxylase, Lauric acid omega hydroxylase

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 11688-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11688-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, rat kidney tissue, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:300-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

11688-1-AP targets CYP4A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP4A11 fusion protein Ag2300
• Full Name: cytochrome P450, family 4, subfamily A, polypeptide 11
• Calculated Molecular Weight: 519 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022851
• Gene Symbol: CYP4A11
• Gene ID (NCBI): 1579
• RRID: AB_10804774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
rat	WB	Food Funct; Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
rat	WB	Food Funct; Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
human	WB	Forensic Sci Int; Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia.; Authors - Liujun Han
mouse,rat	WB	Chem Biol Interact; Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy.; Authors - Shumei Ding