CYP4F11 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP4F11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12286-1-AP

Synonyms

EC:1.14.14.78, EC:1.14.14.1, Docosahexaenoic acid omega-hydroxylase, Cytochrome P450 4F11, CYPIVF11

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Validation Data Gallery

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  WB analysis of human liver using 12286-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12286-1-AP

  WB result of CYP4F11 antibody (12286-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F11 transfected MCF-7 cells.

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  WB analysis using 12286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12286-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12286-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 12286-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 12286-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CYP4F11 antibody (12286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, human liver tissue, human kidney tissue, MCF-7 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

12286-1-AP targets CYP4F11 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP4F11 fusion protein Ag2906
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 11
• Calculated Molecular Weight: 524 aa, 60 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC016853
• Gene Symbol: CYP4F11
• Gene ID (NCBI): 57834
• RRID: AB_2088407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)

Publications

Species	Application	Title
human	WB	Drug Metab Dispos; Gene regulation of CYP4F11 in human keratinocyte HaCaT cells.; Authors - Wang Ying Y
human	ChIP	Drug Metab Dispos; Regulation of cytochrome P450 4F11 by nuclear transcription factor-κB.; Authors - Bell Jordan C JC