CYP4F8 Polyclonal antibody

CYP4F8 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20011-1-AP

Synonyms

CPF8, CYP4F8, CYPIVF8, Cytochrome P450 4F8

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Validation Data Gallery

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  IHC staining of human skin using 20011-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 20011-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 20011-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 20011-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 20011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human skin tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20011-1-AP targets CYP4F8 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 8
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_007253
• Gene Symbol: CYP4F8
• Gene ID (NCBI): 11283
• RRID: AB_2878634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F8, also named as CPF8, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F8 hydroxylates arachidonic acid (20:4n-6) to (18R)-hydroxyarachidonate. CYP4F8 shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. CYP4F8 catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3-hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). It catalyzes hydrxylation of PGI2 and carbaprostacyclin. The antibody has no cross reaction to other CYP4F member