Tested Applications
| Positive WB detected in | MDA-MB-453s cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20142-1-AP targets CYP4Z1-Specific in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cytochrome P450, family 4, subfamily Z, polypeptide 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | NM_178134 |
| Gene Symbol | CYP4Z1 |
| Gene ID (NCBI) | 199974 |
| RRID | AB_10666432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86W10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYP4Z1 belongs to the cytochrome P450 family. CYP4Z1 catalyzes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. This antibody is specific to CYP4Z1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP4Z1-Specific antibody 20142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYP4Z1-Specific Polyclonal antibody (Cat# 20142-1-AP) has 2 total citations. These citations appear in Environmental Toxicology and Molecular Carcinogenesis. Both studies focus on breast cancer research, specifically investigating how targeting CYP4Z1 attenuates stemness in breast cancer cells using WB applications in human samples.
| Species | Application | Title |
|---|---|---|
Environ Toxicol 1-Benzylimidazole attenuates the stemness of breast cancer cells through partially targeting CYP4Z1 | ||
Mol Carcinog HET0016 attenuates the stemness of breast cancer cells through targeting CYP4Z1. |



