CYP4Z1-Specific Polyclonal antibody

CYP4Z1-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20142-1-AP

Synonyms

CYP4A20, CYP4Z1, CYPIVZ1, Cytochrome P450 4Z1

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 20142-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20142-1-AP (CYP4Z1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20142-1-AP (CYP4Z1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20142-1-AP targets CYP4Z1-Specific in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 4, subfamily Z, polypeptide 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: NM_178134
• Gene Symbol: CYP4Z1
• Gene ID (NCBI): 199974
• RRID: AB_10666432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4Z1 belongs to the cytochrome P450 family. CYP4Z1 catalyzes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. This antibody is specific to CYP4Z1.

Publications

Species	Application	Title
human	WB	Environ Toxicol; 1-Benzylimidazole attenuates the stemness of breast cancer cells through partially targeting CYP4Z1; Authors - Hai Qin
human	WB	Mol Carcinog; HET0016 attenuates the stemness of breast cancer cells through targeting CYP4Z1.; Authors - Hai Liu