CYP7B1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82927-1-RR (same clone as 82927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82927-1-RR (CYP7B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82927-1-RR (same clone as 82927-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 82927-1-RR (same clone as 82927-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82927-1-RR (same clone as 82927-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-1-RR, Clone:230160H5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

CYP7B1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82927-1-PBS

Clone No.230160H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230160H5, 24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3

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Product Information

82927-1-PBS targets CYP7B1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19374

  Product name: Recombinant human CYP7B1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 96-403 aa of BC136574

  Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD

• Full Name: cytochrome P450, family 7, subfamily B, polypeptide 1
• Calculated Molecular Weight: 506 aa, 58 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC136574
• Gene Symbol: CYP7B1
• Gene ID (NCBI): 9420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75881
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.