CYP8B1 Recombinant antibody

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  WB analysis using 84909-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84909-1-RR (CYP8B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 84909-1-RR

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 84909-1-RR (CYP8B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CYP8B1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84909-1-RR

Clone No.242086E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242086E12, CP8B, CYP12

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Tested Applications

• Positive WB detected in: HepG2 cells, SMMC-7721 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84909-1-RR targets CYP8B1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 8, subfamily B, polypeptide 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: NM_004391
• Gene Symbol: CYP8B1
• Gene ID (NCBI): 1582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UNU6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 8B1 (CYP8B1) , also named as CP8B, CYP12 and CYPVIIIB1, is an enzyme required for the synthesis of cholic acid (CA) in the classic bile acid synthesis pathway. CYP8B1 controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids.