CYR61 Monoclonal antibody

CYR61 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

2E11F11

Cat no : 67656-1-Ig

Synonyms

CCN family member 1, CCN1, CYR61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1



Tested Applications

Positive WB detected inU2OS cells, A431 cells, PC-3 cells, HeLa cells, MDA-MB-231 cells
Positive IHC detected inhuman liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67656-1-Ig targets CYR61 in WB, IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYR61 fusion protein Ag25129
Full Name cysteine-rich, angiogenic inducer, 61
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC001271
Gene symbol CYR61
Gene ID (NCBI) 3491
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYR61 antibody 67656-1-IgDownload protocol
IHC protocol for CYR61 antibody 67656-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol

Inhibition of Ras protein activator like 2 produces antitumor effects in gastric cancer via the suppression of YAP1 activation

Authors - Yuan Hu
human,mouseWB

Hum Cell

Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity.

Authors - Yanzi Zang