CYR61 Monoclonal antibody

CYR61 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2E11F11

Cat no : 67656-1-Ig

Synonyms

CCN family member 1, CCN1, CYR61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1

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Validation Data Gallery

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  WB analysis using 67656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67656-1-Ig (CYR61 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67656-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67656-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67656-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67656-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 67656-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 67656-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U2OS cells, A431 cells, PC-3 cells, HeLa cells, MDA-MB-231 cells
• Positive IHC detected in: human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67656-1-Ig targets CYR61 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYR61 fusion protein Ag25129
• Full Name: cysteine-rich, angiogenic inducer, 61
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC001271
• Gene Symbol: CYR61
• Gene ID (NCBI): 3491
• RRID: AB_2882854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Hum Cell; Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity.; Authors - Yanzi Zang
human	WB	Environ Toxicol; Inhibition of Ras protein activator like 2 produces antitumor effects in gastric cancer via the suppression of YAP1 activation; Authors - Yuan Hu