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CYTB Polyclonal antibody

CYTB Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55090-1-AP

Synonyms

COB, Complex III subunit 3, Complex III subunit III, CYTB, cytochrome b, MT CYB, MTCYB, MT-CYTB



Tested Applications

Positive WB detected inHepG2 cells, MDA-MB-231 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55090-1-AP targets CYTB in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome b
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberYP_003024038
Gene Symbol CYTB
Gene ID (NCBI) 4519
RRIDAB_2881266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It's mitochondrion membrane protein with MW 43 kDa.

Protocols

Product Specific Protocols
WB protocol for CYTB antibody 55090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Authors - Yi-Ping Wang
humanWB

Cell Rep Med

End binding-3 inhibitor activates regenerative program in age-related macular degeneration

Authors - Quinn Lee
humanWB

Nat Commun

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Authors - Uwe Richter
mouseWB

Nat Commun

Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.

Authors - Vaibhav Sidarala
humanWB

Nat Commun

Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly.

Authors - Shan Zhang
humanWB

Nucleic Acids Res

A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.

Authors - Zidong Jia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mi (Verified Customer) (02-10-2023)

Works very well in differentiated human white adipocyte cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: adipocytes