Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55090-1-AP targets MT-CYB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cytochrome b |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | YP_003024038 |
| Gene Symbol | CYTB |
| Gene ID (NCBI) | 4519 |
| RRID | AB_2881266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00156 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It's mitochondrion membrane protein with MW 43 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MT-CYB antibody 55090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MT-CYB Polyclonal antibody (Cat# 55090-1-AP) has 143 citations in journals including Nature, Cell, Nature Communications, Cell Metabolism, Molecular Cell, Nucleic Acids Research, Journal of Biological Chemistry, and iScience. Research fields span mitochondrial biology and translation, oxidative stress, cancer metabolism, neurodegeneration, cardiovascular disease, diabetes, and hearing loss. The antibody is predominantly used for Western blot, with additional applications in IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Cell Systematic identification of anticancer drug targets reveals a nucleus-to-mitochondria ROS-sensing pathway | ||
Cell PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma | ||
Cell Dynamic mapping of proteome trafficking within and between living cells by TransitID |
Reviews
What customers say
Customers consistently rate this antibody highly for Western blot applications across multiple cell types, including primary skin fibroblasts, lymphoblastoid cells, human primary fibroblasts, and differentiated adipocytes. Most reviewers report strong, reliable performance at 1:1000–1:2000 dilutions. One reviewer noted a light non-specific band, while another suggested that a higher concentration might improve signal intensity. Overall, the antibody is well-received as a dependable WB reagent with good specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alima (Verified Customer) (10-13-2026) | Very good antibody for western blot.
|
Baptiste (Verified Customer) (05-23-2025) | Working well but have a light unspecific band
 |
Yan (Verified Customer) (10-21-2024) | Worked fine in WB assay. Perhaps I should've used more concentrated.
 |
Mi (Verified Customer) (02-10-2023) | Works very well in differentiated human white adipocyte cells.
|
