CYTL1 Polyclonal antibody

CYTL1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15856-1-AP

Synonyms

C17, C4orf4, CYTL1, cytokine like 1, Cytokine like protein 1, Protein C17

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Validation Data Gallery

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  IHC staining of mouse lung using 15856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15856-1-AP (CYTL1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 15856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15856-1-AP (CYTL1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15856-1-AP (CYTL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15856-1-AP (CYTL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse lung tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

15856-1-AP targets CYTL1 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYTL1 fusion protein Ag3977
• Full Name: cytokine-like 1
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: BC031391
• Gene Symbol: CYTL1
• Gene ID (NCBI): 54360
• RRID: AB_2292620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.; Authors - Yun Zhang
mouse	IF	J Bone Miner Res; LPS-induced Inflammation Prior to Injury Exacerbates the Development of Post-Traumatic Osteoarthritis in Mice.; Authors - Melanie E Mendez
mouse	IF	Cells; Single-Cell RNA-Seq Reveals Transcriptomic Heterogeneity and Post-Traumatic Osteoarthritis-Associated Early Molecular Changes in Mouse Articular Chondrocytes.; Authors - Aimy Sebastian
mouse	IHC	Int J Mol Sci; Global Gene Expression Analysis Identifies Age-Related Differences in Knee Joint Transcriptome during the Development of Post-Traumatic Osteoarthritis in Mice.; Authors - Aimy Sebastian
mouse	IF	JBMR Plus; Preexisting Type 1 Diabetes Mellitus Blunts the Development of Posttraumatic Osteoarthritis.; Authors - Naiomy D Rios-Arce
human	IHC	Front Immunol; A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma; Authors - Muhammad Khan