CYTSA Polyclonal antibody

CYTSA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25390-1-AP

Synonyms

cytospin A, CYTSA, KIAA0376, SPECC1 like protein, SPECC1L

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 25390-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25390-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25390-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 25390-1-AP

  IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 25390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, mouse brain tissue, Neuro-2a cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

The immunogen of 25390-1-AP is CYTSA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYTSA fusion protein Ag21896
• Full Name: cytospin A
• Calculated Molecular Weight: 1117 aa, 125 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136471
• Gene Symbol: CYTSA
• Gene ID (NCBI): 23384
• RRID: AB_2880056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.

Publications

Species	Application	Title
mouse	WB,IF	Hum Mol Genet; In-frame deletion of SPECC1L microtubule association domain results in gain-of-function phenotypes affecting embryonic tissue movement and fusion events.; Authors - Jeremy P Goering
human	WB,IF	Hum Mol Genet; SPECC1L regulates palate development downstream of IRF6.; Authors - Everett G Hall
human	WB	Curr Biol; EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules; Authors - Takashi Hotta
human	WB,IF,IP	J Biol Chem; SPECC1L binds the myosin phosphatase complex MYPT1/PP1β and can regulate its distribution between microtubules and filamentous actin; Authors - Virja Mehta