CYTSA Polyclonal antibody

CYTSA Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 25390-1-AP

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

cytospin A, CYTSA, KIAA0376, SPECC1 like protein, SPECC1L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of NIH/3T3 using 25390-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 25390-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 25390-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Neuro-2a using 25390-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 25390-1-AP
IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25390-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25390-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, HeLa cells, mouse brain tissue, Neuro-2a cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25390-1-AP targets CYTSA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21896 Product name: Recombinant human CYTSA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC136471 Sequence: MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEV Predict reactive species
Full Name	cytospin A
Calculated Molecular Weight	1117 aa, 125 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC136471
Gene Symbol	CYTSA
Gene ID (NCBI)	23384
RRID	AB_2880056
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q69YQ0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.

Protocols

Product Specific Protocols
IHC protocol for CYTSA antibody 25390-1-AP	Download protocol
IP protocol for CYTSA antibody 25390-1-AP	Download protocol
WB protocol for CYTSA antibody 25390-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-SPECC1L antibody (Cat# 25390-1-AP) has 8 total citations. These appear in Current Biology, Frontiers in Physiology, Journal of Biological Chemistry, Human Molecular Genetics, and bioRxiv. Research fields include cytoskeletal biology (microtubule dynamics, actin regulation), developmental biology (cranial neural crest, palate development, frontonasal dysplasia), and signaling pathway regulation (Hedgehog signaling, IRF6 downstream effects).

Species	Application	Title
human	WB	Curr Biol EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules Authors - Takashi Hotta View Article
mouse	IF	Front Physiol Loss of SPECC1L in cranial neural crest cells results in increased hedgehog signaling and frontonasal dysplasia. Authors - An J. Tran View Article
human	WB,IF,IP	J Biol Chem SPECC1L binds the myosin phosphatase complex MYPT1/PP1β and can regulate its distribution between microtubules and filamentous actin Authors - Virja Mehta View Article
human	WB,IF	Hum Mol Genet SPECC1L regulates palate development downstream of IRF6. Authors - Everett G Hall View Article
mouse	WB,IF	Hum Mol Genet In-frame deletion of SPECC1L microtubule association domain results in gain-of-function phenotypes affecting embryonic tissue movement and fusion events. Authors - Jeremy P Goering View Article
mouse	IF	bioRxiv Loss of SPECC1L in cranial neural crest cells results in increased hedgehog signaling and frontonasal dysplasia. Authors - An J Tran View Article