Cadherin-17 Recombinant monoclonal antibody

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  WB analysis of mouse colon using 83793-5-RR

  mouse colon tissue was subjected to SDS PAGE followed by western blot with 83793-5-RR (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83793-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83793-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83793-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 83793-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 83793-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 83793-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human stomach cancer using 83793-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human stomach cancer using 83793-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human stomach cancer using 83793-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83793-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83793-5-RR against Human Cadherin-17 were performed. The affinity constant is below 1 pM.

Cadherin-17 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83793-5-RR

Clone No.240681B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IHC detected in: human stomach cancer tissue, mouse colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human stomach cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83793-5-RR targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19488

  Product name: Recombinant human CDH17 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 89-406 aa of BC113464

  Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA

• Full Name: cadherin 17, LI cadherin (liver-intestine)
• Calculated Molecular Weight: 832 aa, 92 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC113464
• Gene Symbol: Cadherin-17
• Gene ID (NCBI): 1015
• RRID: AB_3671384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q12864
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.