Caspase 4 Recombinant monoclonal antibody

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• Caspase 4 Recombinant monoclonal antibody (88257-2-RR)

Caspase 4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88257-2-RR Copied!

Clone No. 260526A10

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CASP4, Caspase4, CASP 4, CASP-4, Caspase 4/p20/p10

• 

  WB analysis using 88257-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88257-2-RR (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 88257-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88257-2-RR (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: THP-1 cells, RAW 264.7 cells, SGC-7901 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88257-2-RR targets Caspase 4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 4, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43-48 kDa
• GenBank Accession Number: BC017839
• Gene Symbol: Caspase 4
• Gene ID (NCBI): 837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49662
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.