Caspase 6 Recombinant antibody

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  WB analysis of Jurkat using 85201-2-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85201-2-RR (Caspase 6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Caspase 6 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85201-2-RR

Clone No.242457A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CASP6, Caspase6, 242457A8, Apoptotic protease Mch 2, Apoptotic protease Mch-2

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85201-2-RR targets Caspase 6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 6, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 33 kDa, 22 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000305
• Gene Symbol: Caspase 6
• Gene ID (NCBI): 839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55212
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons.