Cathepsin L Polyclonal antibody

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  WB analysis of A549 using 27952-1-AP

  WB result of Cathepsin L antibody (27952-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

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  WB analysis using 27952-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27952-1-AP (Cathepsin L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27952-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27952-1-AP (Cathepsin L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27952-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27952-1-AP (Cathepsin L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Cathepsin L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27952-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

27952-1-AP targets Cathepsin L in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27813

  Product name: Recombinant human Cathepsin L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 114-288 aa of BC012612

  Sequence: APRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFEST

• Full Name: cathepsin L1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 36-39 kDa, 29 kDa
• GenBank Accession Number: BC012612
• Gene Symbol: Cathepsin L
• Gene ID (NCBI): 1514
• RRID: AB_2881021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07711
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Front Pharmacol; Nodosin Exerts an Anti-Colorectal Cancer Effect by Inhibiting Proliferation and Triggering Complex Cell Death in Vitro and in Vivo; Authors - Huixia Fan
human	WB	Acta Pharmacol Sin; Salvianolic acid B suppresses hepatic fibrosis by inhibiting ceramide glucosyltransferase in hepatic stellate cells; Authors - Zi-Bo Li
human	IHC	Transl Oncol; Mitochondria-related chemoradiotherapy resistance genes-based machine learning model associated with immune cell infiltration on the prognosis of esophageal cancer and its value in pan-cancer; Authors - Ziyu Liu
mouse	WB	J Med Chem; Design, Synthesis, and Biological Evaluation of 2-Arylaminopyrimidine Derivatives as Dual Cathepsin L and JAK Inhibitors for the Treatment of Acute Lung Injury; Authors - Chunwei Shen
human	WB	Pharmacol Res; The molecular receptor NKBB enhances the persistence and anti-hepatocellular carcinoma activity of GPC3 CAR-T cells; Authors - Minghao Sui
human	WB,IHC,IF	World J Gastroenterol; Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer; Authors - Lu-Xi Xiao ; KD Validated