Tested Applications
| Positive WB detected in | A549 cells, NCI-H1299 cells, HUVEC cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66914-1-Ig targets Cathepsin L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27951 Product name: Recombinant human Cathepsin L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-333 aa of BC012612 Sequence: MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNVKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV Predict reactive species |
| Full Name | cathepsin L1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC012612 |
| Gene Symbol | Cathepsin L |
| Gene ID (NCBI) | 1514 |
| RRID | AB_2882241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cathepsin L antibody 66914-1-Ig | Download protocol |
| IHC protocol for Cathepsin L antibody 66914-1-Ig | Download protocol |
| WB protocol for Cathepsin L antibody 66914-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cathepsin L Monoclonal antibody (Cat No. 66914-1-Ig) has 7 total citations. These appear in journals including Molecular Cancer, Clinical Science, Cancer Science, Human Genomics, Tissue and Cell, Chemistry & Biodiversity, and Physical Activity and Nutrition. The associated research fields span pancreatic cancer, adipose browning, cervical cancer metastasis, atherosclerotic plaque vulnerability, pressure injury, colorectal cancer, and cardiac catabolism during chemotherapy. Applications cited include Western blot, immunofluorescence, and immunohistochemistry.
| Species | Application | Title |
|---|---|---|
Mol Cancer Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer | ||
Clin Sci (Lond) Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning | ||
Cancer Sci Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner
| ||
Hum Genomics APM⁺ macrophages associated with plaque vulnerability via MIF-CD74 signaling: a multi-omics study. | ||
Tissue Cell High expression of SERPINE1 and CTSL in keratinocytes in pressure injury caused by ischemia-reperfusion injury | ||
Chem Biodivers Design, Synthesis and Biological Evaluation of Naphthalimide-Polyamine Conjugate as a Potential Anti-Colorectal Cancer Agent |
