Cathepsin L Monoclonal antibody

• Featured Product
• KD/KO Validated

Cathepsin L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1A12G8

Cat no : 66914-1-Ig

Synonyms

CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain

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Validation Data Gallery

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  WB analysis of A549 using 66914-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66914-1-Ig

  WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

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  WB analysis of HeLa using 66914-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 66914-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 66914-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66914-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66914-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66914-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HEK-293 using 66914-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: A549 cells, NCI-H1299 cells, HUVEC cells, HepG2 cells, HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

66914-1-Ig targets Cathepsin L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cathepsin L fusion protein Ag27951
• Full Name: cathepsin L1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC012612
• Gene Symbol: Cathepsin L
• Gene ID (NCBI): 1514
• RRID: AB_2882241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Publications

Species	Application	Title
human	WB	Mol Cancer; Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer; Authors - Cheng-Ke Xie
mouse	WB	Clin Sci (Lond); Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning; Authors - Limin Shi
human	WB	Cancer Sci; Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner; Authors - Pingping Liu ; KD Validated