Cd68 Polyclonal antibody

Cd68 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (3)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

184

Reviews (2)

Cat no : 28058-1-AP

Synonyms

Cd68, CD68 antigen, gp110, Scard1

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 28058-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 28058-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 28058-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Mouse Cd68 (28058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse spleen tissue
• Positive FC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:600-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 37 publications below
• IHC: See 78 publications below
• IF: See 78 publications below
• FC: See 2 publications below

Product Information

28058-1-AP targets Cd68 in WB, IF, FC, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cd68 fusion protein Ag27827
• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001291058
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• RRID: AB_2881049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	IF	J Pineal Res; Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.; Authors - Wei Liu
mouse	IF	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
	IF	J Exp Clin Cancer Res; FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression; Authors - Wei-Min Hu
mouse	IF	Acta Biomater; Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization; Authors - Yongzheng Li
mouse	IHC	Biomaterials; Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.; Authors - Ting Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandru (Verified Customer) (11-13-2023)

Great antibody, worked well in my experiments.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse ventricle

FH

Amelie (Verified Customer) (09-09-2019)

sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse adrenal gland

Cd68 Antibody Immunofluorescence, validation (1:1000 dilution) in mouse adrenal gland (Cat no:28058-1-AP)