Chordin Polyclonal antibody, PBS Only

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• Chordin Polyclonal antibody, PBS Only (27293-1-PBS)

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  WB analysis of 3T3-L1 using 27293-1-AP (same clone as 27293-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27293-1-AP (CHRD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 27293-1-AP (same clone as 27293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 27293-1-AP (same clone as 27293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

Chordin Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27293-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

27293-1-PBS targets Chordin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26001

  Product name: Recombinant human CHRD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-350 aa of BC112345

  Sequence: AGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQA

• Full Name: chordin
• Calculated Molecular Weight: 955 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC112345
• Gene Symbol: Chordin
• Gene ID (NCBI): 8646
• RRID: AB_2880832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2X0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration.