Tested Applications
| Positive WB detected in | HuH-7 cells |
| Positive IHC detected in | human endometrial cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30520-1-AP targets Claudin 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29607 Product name: Recombinant human CLDN9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 163-217 aa of BC051870 Sequence: SLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV Predict reactive species |
| Full Name | claudin 9 |
| Calculated Molecular Weight | 217 aa, 23 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC051870 |
| Gene Symbol | Claudin 9 |
| Gene ID (NCBI) | 9080 |
| RRID | AB_3086347 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95484 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Claudin 9 antibody 30520-1-AP | Download protocol |
| IHC protocol for Claudin 9 antibody 30520-1-AP | Download protocol |
| WB protocol for Claudin 9 antibody 30520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Claudin 9 Polyclonal antibody (Cat# 30520-1-AP) has 1 total citation. It appears in the International Neurourology Journal (2025), in a study examining neurovascular restoration by treadmill exercise to attenuate age-related cognitive decline in mice. The cited application was Western blot. The research field is neuroscience, specifically neurovascular biology and cognitive aging.
| Species | Application | Title |
|---|---|---|
Int Neurourol J Neurovascular Restoration by Treadmill Exercise Attenuates Age-Related Cognitive Decline in Mice. |
