Cleaved Caspase 2/P12 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• Cleaved Caspase 2/P12 Recombinant monoclonal antibody (88209-2-RR)

Cleaved Caspase 2/P12 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88209-2-RR

Clone No.253365E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CASP2, Caspase 2, CASP 2, CASP-2, Caspase 2/p18

• 

  WB analysis of Jurkat using 88209-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88209-2-RR (Cleaved Caspase 2/P12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88209-2-RR targets Cleaved Caspase 2/P12 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 2, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC002427
• Gene Symbol: Caspase 2
• Gene ID (NCBI): 835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42575
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Caspase 2 is synthesized in its inactive prozymogen form and is activated upon stimulation by apoptosis signals. During activation, the prozymogen is cleaved at a specific site, producing a smaller p12 subunit and a larger p18 subunit. The p12-p18 tetramer structure is the core form in which Caspase 2 functions.