Cleaved Caspase 3/P17 Recombinant antibody, PBS Only (Detector)

Cleaved Caspase 3/P17 Recombinant Antibody for Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230527H5

Cat no : 82707-11-PBS

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Validation Data Gallery

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  Standard curve of MP00104-2

  Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

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  Standard curve of MP00104-3

  Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82707-11-PBS targets Cleaved Caspase 3/P17 as part of a matched antibody pair:

MP00104-2: 82707-10-PBS capture and 82707-11-PBS detection (validated in Cytometric bead array)

MP00104-3: 82707-8-PBS capture and 82707-11-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D=4.78 x 10^-9M
  K_Off=2.19 x 10^-4M
  K_On=4.59 x 10^5M
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_032991
• Gene Symbol: CASP3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.