Cleaved Caspase 3/P17/P19 Recombinant monoclonal antibody, PBS Only

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• Cleaved Caspase 3/P17/P19 Recombinant monoclonal antibody, PBS Only (82707-13-PBS)

Cleaved Caspase 3/P17/P19 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 82707-13-PBS Copied!

Clone No. 230527B2

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CASP3, Caspase3, 230527B2, CASP 3, CASP-3

• 

  WB analysis using 82707-13-RR (same clone as 82707-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82707-13-RR (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of Jurkat using 82707-13-RR (same clone as 82707-13-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 82707-13-RR (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Jurkat using 82707-13-RR (same clone as 82707-13-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Staurosporine treated Jurkat cells using Cleaved Caspase 3/P17/P19 antibody (82707-13-RR, Clone: 230527B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

82707-13-PBS targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 17 kDa, 19 kDa
• GenBank Accession Number: NM_032991
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• RRID: AB_3670535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42574
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publications

What published studies show

The Cleaved Caspase 3/P17/P19 Recombinant Monoclonal Antibody (catalog number 82707-13-PBS) has 1 total citation. It appears in the journal Phytotherapy Research (impact factor 8.1), in a 2025 study by Zimeng Wang titled "Oridonin Ameliorates Metabolic-Dysfunction-Associated Fatty Liver Disease by Inhibiting PANoptosis via Modulating SIRT2/NLRP3 Pathway." The research field is metabolic dysfunction-associated fatty liver disease and PANoptosis, using Western blot in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Phytother Res; Oridonin Ameliorates Metabolic-Dysfunction-Associated Fatty Liver Disease by Inhibiting PANoptosis via Modulating SIRT2/NLRP3 Pathway; Authors - Zimeng Wang