Cleaved N-terminal GSDMD (Asp275) Polyclonal antibody

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• Cleaved N-terminal GSDMD (Asp275) Polyclonal antibody (33825-1-AP)

Cleaved N-terminal GSDMD (Asp275) Polyclonal Antibody for WB, ELISA

Cat No. 33825-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cleaved Gasdermin D, Cleaved Gasdermin D (Asp275), Cleaved GSDMD, Cleaved GSDMD (Asp275), Cleaved N-terminal GSDMD

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  WB analysis using 33825-1-AP

  Untreated, PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 33825-1-AP (Cleaved GSDMD (Asp275) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33825-1-AP targets Cleaved N-terminal GSDMD (Asp275) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: gasdermin D
• Calculated Molecular Weight: 484 aa, 53 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC008904
• Gene Symbol: GSDMD
• Gene ID (NCBI): 79792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P57764
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gasdermin D (GSDMD) is a two‑domain cytosolic protein that acts as the principal effector of pyroptosis. In its full‑length, uncleaved state, GSDMD adopts an autoinhibited conformation in which the C‑terminal domain (CT‑GSDMD) binds and suppresses the pore‑forming N‑terminal domain (NT‑GSDMD), thereby preventing premature membrane damage. Upon activation of canonical or non‑canonical inflammasomes, inflammatory caspases (primarily caspase‑1, ‑4, and ‑5 in humans) specifically cleave GSDMD at the aspartate residue 275 (Asp275) within the flexible interdomain linker. This proteolytic event separates the inhibitory CT‑domain from the NT‑domain, yielding the active cleaved GSDMD (Asp275) fragment-commonly referred to as NT‑GSDMD p30. The liberated N‑terminal fragment then translocates to the plasma membrane, oligomerizes, and inserts into the lipid bilayer to form large β‑barrel pores, which disrupt osmotic homeostasis, cause cell swelling and lysis, and facilitate the release of pro‑inflammatory cytokines such as IL‑1β and IL‑18. Thus, while full‑length GSDMD serves as a dormant guardian that maintains cellular integrity, cleaved GSDMD at Asp275 represents the irreversible executioner that triggers inflammatory cell death, underscoring Asp275 as the critical molecular switch that governs pyroptotic signaling.