Coagulation Factor II Polyclonal antibody

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  WB analysis using 31615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 31615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 31615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 31615-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Coagulation Factor II antibody (31615-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Coagulation Factor II Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31615-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, PT

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Tested Applications

• Positive WB detected in: human plasma, mouse heart tissue, rat plasma, mouse liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31615-1-AP targets Coagulation Factor II in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: coagulation factor II
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_010168.3
• Gene Symbol: F2
• Gene ID (NCBI): 14061
• RRID: AB_3670049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19221
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis. This antibody specifically recognizes mouse Coagulation Factor II.