COL4A1 Polyclonal antibody

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  WB analysis using 30850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30850-1-AP (COL4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 30850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 30850-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 30850-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 30850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30850-1-AP (Collagen Type IV antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COL4A1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30850-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

arresten, Collagen alpha 1(IV) chain, Collagen alpha-1(IV) chain, Collagen Type IV

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Tested Applications

• Positive WB detected in: HeLa cells, SK-N-SH cells
• Positive IHC detected in: human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

30850-1-AP targets COL4A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COL4A1 fusion protein Ag34391
• Full Name: collagen, type IV, alpha 1
• Calculated Molecular Weight: 1669 aa, 161 kDa
• Observed Molecular Weight: 160-220 kDa
• GenBank Accession Number: BC151220
• Gene Symbol: COL4A1
• Gene ID (NCBI): 1282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P02462
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL4A1 (Collagen Type IV Alpha 1 Chain) encodes a major component of type IV collagen, which is an essential structural element of basement membranes in various tissues. COL4A1 forms heterotrimers with other collagen type IV chains (e.g., COL4A2) and interacts with extracellular matrix components such as laminins, proteoglycans, and perlecans. Proteolytic cleavage of the non-collagenous domain of COL4A1 produces a fragment called arresten, which has anti-angiogenic and tumor suppressor properties. Elevated expression of COL4A1 has been observed in various cancers, including hepatocellular carcinoma (HCC), where it promotes tumor growth and metastasis through the FAK-Src signaling pathway. COL4A1 is highly expressed in various tissues, including the placenta, fat, and other organs. The expression of COL4A1 can be regulated by transcription factors such as RUNX1, which has been shown to upregulate COL4A1 in hepatocellular carcinoma. The molecular weight of OSBPL5 is 160 kDa, and it also has glycosylation modification.

Publications

Species	Application	Title
mouse	IHC	Sci Rep; Yiqi Juanshen decoction alleviates renal interstitial fibrosis by targeting the LOXL2/PI3K/AKT pathway to suppress EMT and inflammation; Authors - Kaiyue Tan