Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse lung tissue, rat lung tissue|
|Positive IHC detected in||human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human kidney tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55131-1-AP targets COL4A2-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||collagen, type IV, alpha 2|
|Calculated molecular weight||168 kDa|
|Observed molecular weight||200 kDa|
|GenBank accession number||NM_001846|
|Gene ID (NCBI)||1284|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Pankhuri (Verified Customer) (08-22-2022)
Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.
Lindsey (Verified Customer) (05-24-2021)
Malak (Verified Customer) (02-24-2021)
This antibody works very well
Joshua (Verified Customer) (04-28-2019)
Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.