Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55131-1-AP targets COL4A2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | collagen, type IV, alpha 2 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_001846 |
| Gene Symbol | COL4A2 |
| Gene ID (NCBI) | 1284 |
| RRID | AB_2881273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08572 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COL4A2 antibody 55131-1-AP | Download protocol |
| IHC protocol for COL4A2 antibody 55131-1-AP | Download protocol |
| WB protocol for COL4A2 antibody 55131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COL4A2 Polyclonal antibody (Cat# 55131-1-AP) has 85 citations. Publications appear in journals including Gastroenterology, J Clin Invest, EMBO J, Biomaterials, Cell Death Dis, Matrix Biology, and Front Pharmacol, among others. Research fields span renal and liver fibrosis, diabetic nephropathy, extracellular matrix remodeling, cancer metastasis (breast, liver, lung, bladder), vascular biology, tissue engineering, inflammation, and ophthalmology. Applications include WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Gastroenterology Macrophage-derived cathepsin S remodels the extracellular matrix to promote liver fibrogenesis | ||
J Exp Clin Cancer Res Therapeutic synergies that overcome carboplatin resistance in triple-negative breast cancer. | ||
J Clin Invest Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma | ||
Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
Cell Death Dis LincRNA-Gm4419 knockdown ameliorates NF-κB/NLRP3 inflammasome-mediated inflammation in diabetic nephropathy. | ||
Cell Death Dis Methyl-CpG-binding domain protein 2 contributes to renal fibrosis through promoting polarized M1 macrophages. |
Reviews
What customers say
Customers consistently rate this antibody highly (4–5 stars) across multiple applications. Western blot users report clean, specific bands in mouse dorsal root ganglion and spinal cord tissue. Immunofluorescence reviewers praise its performance in NSC organoids and 3D vascular endothelial cell cultures, noting crisp collagen IV staining at basement membranes. One IHC user on human kidney FFPE described it as "bulletproof." Overall, reviewers highlight strong specificity, low background, and reliable results at dilutions ranging from 1:500 to 1:5000.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Samantha (Verified Customer) (05-10-2024) | Clean bands detected in DRG and Spinal cord tissue of mice.
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Pankhuri (Verified Customer) (08-22-2022) | Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.
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Lindsey (Verified Customer) (05-24-2021) | BULLETPROOF ANTIBODY!
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Malak (Verified Customer) (02-24-2021) | This antibody works very well
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Joshua (Verified Customer) (04-28-2019) | Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.
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