Collagen Type IX Polyclonal antibody

Collagen Type IX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24148-1-AP

Synonyms

COL9A2, Collagen alpha 2(IX) chain, Collagen IX, Collagen Type IX, collagen, type IX, alpha 2, EDM2, MED

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Validation Data Gallery

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  WB analysis using 24148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24148-1-AP (Collagen Type IX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse ovary tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24148-1-AP targets Collagen Type IX in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type IX fusion protein Ag21458
• Full Name: collagen, type IX, alpha 2
• Calculated Molecular Weight: 689 aa, 65 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC136326
• Gene Symbol: COL9A2
• Gene ID (NCBI): 1298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. This chain is unusual in that, unlike the other two type IX alpha chains, it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia.