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Collagen Type XI Polyclonal antibody, PBS Only (21841-1-PBS)

Filter: AllWBIHCIF-P

WB analysis using 21841-1-AP (same clone as 21841-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

Collagen Type XI Polyclonal antibody, PBS Only

Name: Collagen Type XI Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21841-1-PBS

Collagen Type XI Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 21841-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21841-1-PBS targets Collagen Type XI in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16509 Product name: Recombinant human COL11A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1429-1690 aa of BC117697 Sequence: PLPILSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSGSYDKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQNQKFGFEVGPVCFLG Predict reactive species
Full Name	collagen, type XI, alpha 1
Calculated Molecular Weight	1818 aa, 182 kDa
Observed Molecular Weight	250 kDa, 181 kDa
GenBank Accession Number	BC117697
Gene Symbol	COL11A1
Gene ID (NCBI)	1301
RRID	AB_2918074
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12107
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)