Collagen Type XXVII Polyclonal antibody, PBS Only

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• Collagen Type XXVII Polyclonal antibody, PBS Only (15673-1-PBS)

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  WB analysis using 15673-1-AP (same clone as 15673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15673-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 15673-1-AP (same clone as 15673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15673-1-PBS in a different storage buffer formulation.

Collagen Type XXVII Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 15673-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

15673-1-PBS targets Collagen Type XXVII in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8140

  Product name: Recombinant human COL27A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-329 aa of BC007696

  Sequence: LSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTIIPAALGTELALVLSLCSHRVNHAFLFAVRSRKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPI

• Full Name: collagen, type XXVII, alpha 1
• Calculated Molecular Weight: 1860 aa, 87 kDa
• Observed Molecular Weight: 68-73 kDa
• GenBank Accession Number: BC007696
• Gene Symbol: COL27A1
• Gene ID (NCBI): 85301
• RRID: AB_3085469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZC6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Collagen Type XXVII, also named COL27A1, it is a member of the collagen fibrillar gene family . It is located in the extrcellular matrix. The calculated molecular weight of COL27A1 is 186 kDa. This antibody can recognize the 73 kDa isoform of target. The high level of Col27a1 expression in mouse cartilage suggested the possibility that Col27a1 may be regulated in cartilage by SOX9, as are Col2a1, Col11a2, Col9a1, and Col9a2 (PMID: 15922909). Expression analyses of the mouse col27a1 gene demonstrated high expression in cartilage, the eye and ear, but also in lung and colon. It is likely that the major protein product of COL27A1, proa1(XXVII), is a component of the extracellular matrices of cartilage and these other tissues. Study of this collagen should yield insights into normal chondrogenesis, and provide clues to the pathogenesis of some chondrodysplasias and disorders of other tissues in which this gene is expressed (PMID: 12714037).