Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23161-1-AP targets Connexin-50 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19572 Product name: Recombinant human GJA8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 233-422 aa of BC146507 Sequence: RSALKRPVEQPLGEIPEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLPAKPFNQFEEKISTGPLGDLSRGYQETLPSYAQVGAQEVEGEGPPAEEGAEPEVGEKKEEAERLTTEEQEKVAVPEGEKVETPGVDKEGEKEEPQSEKVSKQGLPAEKTPSLCPELTTDDARPLSRLSKA Predict reactive species |
| Full Name | gap junction protein, alpha 8, 50kDa |
| Calculated Molecular Weight | 433 aa, 48 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC146507 |
| Gene Symbol | GJA8 |
| Gene ID (NCBI) | 2703 |
| RRID | AB_3669410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P48165 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Connexin-50 (Cx50), also known as Gap junction alpha 8 protein (GJA8), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-50 is expressed in lens fiber cells and is essential for the normal physiological function of the lens (PMID: 11916859). Together with Connexin-46, Connexin-50 forms functional half-gap junction channels in non-junctional membranes. The expected molecular weight of Connexin-50 is 50 kDa, and in some literature, Connexin-50 was detected in lens samples at ~65 kDa (PMID: 31195409; PMID: 29196765).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Connexin-50 antibody 23161-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Connexin-50 Polyclonal antibody (Cat# 23161-1-AP) has 1 total citation. It was cited in European Journal of Human Genetics (2023), in a study investigating the association of GJA8 variants with familial acorea-microphthalmia-cataract syndrome. The research field is human genetics and ophthalmology, specifically focusing on gap junction gene variants linked to inherited eye disorders. The antibody was used for immunofluorescence (IF) on human samples.
| Species | Application | Title |
|---|---|---|
Eur J Hum Genet Association of variants in GJA8 with familial acorea-microphthalmia-cataract syndrome |
