CoraLite®488-Annexin V and PI Apoptosis Kit

PI: Ex/Em

535/617 nm

CL488-Annexin V: Ex/Em

490/515 nm

Application

FC, IF

Cat no : PF00005



Product Information

CoraLite®488-Annexin V and PI Apoptosis Detection Kit provides a quick and easy method to detect apoptosis by marking early apoptotic cells (green) and late apoptotic cells or necrotic cells (red).Results can be read out using flow cytometry or other fluorescence testing equipment. Early apoptic cells are identified by annexin V (labeled with CoraLite(R) 488 dye) binding to phosphatidylserine (PS) flipped to the extracellular membrane surface, an early event in apoptosis. Compared with FITC, CoraLite(R) 488 dye has higher fluorescence brightness, and is not affected by pH in the environment, and has good light stability. 

Propidium lodide (PI) is a DNA-binding dye that can stain the nucleus of necrotic cells or cells that lose cell membrane integrity in the late stages of apoptosis. PI can be excited by lasers at 488, 532 or 546nm and CoraLite(R) 488's excitation maximum is 490 nm.


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 Components

50T

100T

 1×Annexin V Binding Buffer

50 mL

50 mL X 2

 CL488-Annexin V

250 μL

500 μL

 PI

250 μL

1 mL


Storage

Store at 4°C,  Avoid exposure to light, DO NOT freeze. stable for 6 months after shipment.

Cautions

1. Please try to avoid light during storage.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For most accurate results, detection must performed as soon as possible after staining.

Spectral Characteristics

CL488-AnnexinV: Ex/Em=490/515nm

PI: Ex/Em=535/617nm (withDNA)