Cortactin Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01412-2

  Sandwich ELISA standard curve of MP01412-2, Human Cortactin Recombinant Matched Antibody Pair - PBS only. 84569-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1940. 84569-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01412-2

  The mean Cortactin concentration was determined to be 848.3 ng/mL in A549 cell extract based on a 2.3 mg/mL extract load, 581.1 ng/mL in HEK-293 cell extract based on a 3.3 mg/mL extract load and 493.0 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP01412-2

  Cytometric bead array standard curve of MP01412-2, Cortactin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84569-2-PBS. Detection antibody: 84569-1-PBS. Standard: Ag1940. Range: 0.313-40 ng/mL.

Cortactin Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84569-2-PBS

Clone No.241987B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CTTN, SRC8, EMS1, 241987B4

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Product Information

84569-2-PBS targets Cortactin as part of a matched antibody pair:

MP01412-2: 84569-2-PBS capture and 84569-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1940

  Product name: Recombinant human Cortactin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 206-513 aa of BC008799

  Sequence: VDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ

• Full Name: cortactin
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: BC008799
• Gene Symbol: Cortactin
• Gene ID (NCBI): 2017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14247
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.